Cardiac expression of skeletal muscle sodium channels increases longitudinal conduction velocity in the canine 1-week myocardial infarction
R Coronel, DH Lau, EA Sosunov, MJ Janse, P Danilo… – Heart Rhythm, 2010
BACKGROUND: Skeletal muscle sodium channel (Nav1. 4) expression in border zone
myocardium increases action potential upstroke velocity in depolarized isolated tissue.
Because resting membrane potential in the 1-week canine infarct is reduced, we …
myocardium increases action potential upstroke velocity in depolarized isolated tissue.
Because resting membrane potential in the 1-week canine infarct is reduced, we …
Experimental ‘jet lag’inhibits adult neurogenesis and produces long-term cognitive deficits in female hamsters
EM Gibson, C Wang, S Tjho, N Khattar, LJ Kriegsfeld – PLoS One, 2010
Background Circadian disruptions through frequent transmeridian travel, rotating shift work,
and poor sleep hygiene are associated with an array of physical and mental health
maladies, including marked deficits in human cognitive function. Despite anecdotal and …
and poor sleep hygiene are associated with an array of physical and mental health
maladies, including marked deficits in human cognitive function. Despite anecdotal and …
Human CD34+ Cells in Experimental Myocardial Infarction Long-Term Survival, Sustained Functional Improvement, and Mechanism of Action
J Wang, S Zhang, B Rabinovich, L Bidaut… – Circulation research, 2010
Abstract Rationale: Human CD34+ cells have been used in clinical trials for treatment of
myocardial infarction (MI). However, it is unknown how long the CD34+ cells persist in
hearts, whether the improvement in cardiac function is sustained, or what are the …
myocardial infarction (MI). However, it is unknown how long the CD34+ cells persist in
hearts, whether the improvement in cardiac function is sustained, or what are the …
Hepatocarcinoma cells stimulate the growth, migration and expression of pro‐angiogenic genes in human hepatic stellate cells
P Sancho‐Bru, E Juez, M Moreno, V Khurdayan… – Liver International, 2010
Background: Activated hepatic stellate cells (HSC) and other fibrogenic cell types are
frequently found around hepatocellular carcinoma. It is unknown whether hepatocarcinoma
cells regulate the biological functions of HSC. Aims: This study aimed to investigate the …
frequently found around hepatocellular carcinoma. It is unknown whether hepatocarcinoma
cells regulate the biological functions of HSC. Aims: This study aimed to investigate the …
In vitro regulation of neural differentiation and axon growth by growth factors and bioactive nanofibers
Human embryonic stem cell (ESC)–derived neural cells are a potential cell source for neural
tissue regeneration. Understanding the biochemical and biophysical regulation of neural
differentiation and axon growth will help us develop cell therapies and bioactive scaffolds. …
tissue regeneration. Understanding the biochemical and biophysical regulation of neural
differentiation and axon growth will help us develop cell therapies and bioactive scaffolds. …
Targeted nonviral delivery vehicles to neural progenitor cells in the mouse subventricular zone
EJ Kwon, J Lasiene, BE Jacobson, IK Park, PJ Horner… – Biomaterials, 2010
Targeted gene therapy can potentially minimize undesirable off-target toxicity due to specific
delivery. Neuron-specific gene delivery in the central nervous system is challenging
because neurons are non-dividing and also outnumbered by glial cells. One approach is …
delivery. Neuron-specific gene delivery in the central nervous system is challenging
because neurons are non-dividing and also outnumbered by glial cells. One approach is …
Sensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detection
AC Mak, SJ Osterfeld, H Yu, SX Wang, RW Davis… – Biosensors and …, 2010
Rapid and multiplexed measurement is vital in the detection of food-borne pathogens. While
highly specific and sensitive, traditional immunochemical assays such as enzyme-linked
immunosorbent assays (ELISAs) often require expensive read-out equipment (eg …
highly specific and sensitive, traditional immunochemical assays such as enzyme-linked
immunosorbent assays (ELISAs) often require expensive read-out equipment (eg …
Resistance of strains of Pseudomonas aeruginosa to contact lens disinfectants. My Citations
C Lakkis, DW Frank, AS Bruce, SMJ Fleiszig – … OPHTHALMOLOGY & VISUAL …, 1997
Resistance of strains of Pseudomonas aeruginosa to contact lens disinfectants. C Lakkis,
DW Frank, AS Bruce, SMJ Fleiszig INVESTIGATIVE OPHTHALMOLOGY & VISUAL
SCIENCE 38:44, 2415-2415, LIPPINCOTT-RAVEN PUBL, 3/1997.
DW Frank, AS Bruce, SMJ Fleiszig INVESTIGATIVE OPHTHALMOLOGY & VISUAL
SCIENCE 38:44, 2415-2415, LIPPINCOTT-RAVEN PUBL, 3/1997.
171 TATG ACT GCG ACA GAC AGA ACG CCC CCG CC ATTG My Citations
MS Hindahl, DW Frank, A Hamood, BH Iglewski – Nucleic Acids Research, 1988
Abstract An incorrect version of the regA sequence was inadvertently printed. The correct
version should have a T residue inserted at position 202, as shown below. As a result, the
start codon for regA resides at position 172 rather than position 201. This changes the …
version should have a T residue inserted at position 202, as shown below. As a result, the
start codon for regA resides at position 172 rather than position 201. This changes the …
Treating bacterial virulence systems: we are not there yet My Citations
YJ Huang, EA Bittner, D Frank, J Wiener-Kronish – Intensive care medicine, 2012
In the manuscript by van Delden and colleagues [1], the claim is made that azithromycin was
successful in inhibiting quorum sensing (QS) and decreased the incidence of Pseudomonas
aeruginosa-ventilator-associated pneumonia (VAP). This conclusion belies the complexity …
successful in inhibiting quorum sensing (QS) and decreased the incidence of Pseudomonas
aeruginosa-ventilator-associated pneumonia (VAP). This conclusion belies the complexity …
cCMP and cUMP occur in vivo My Citations
B Schirmer, U Beckert, DW Frank, B Tümmler… – … and Biophysical Research …, 2015
Q8 Heike Bähre a, b, 1, Christina Hartwig a, 1, Antje Munder c, 1, Sabine Wolter a, Tane Stelzer
a, Bastian Schirmer a, Ulrike Beckert a, Dara W. Frank d, Burkhard Tümmler c, Volkhard Kaever
a, b, Roland Seifert a, * a Institute of Pharmacology, Hannover Medical School, D-30625 …
a, Bastian Schirmer a, Ulrike Beckert a, Dara W. Frank d, Burkhard Tümmler c, Volkhard Kaever
a, b, Roland Seifert a, * a Institute of Pharmacology, Hannover Medical School, D-30625 …
The Pseudomonas AerugINOSa Exotoxin Y Induces Inter-Endothelial Cell Gaps And Impairs Migration And Proliferation My Citations
Pseudomonas aeruginosa is a common cause of illness in immunocompromised patients.
Virulence is due to a type III secretion system (T3SS) that introduces exotoxins into the host
cell cytoplasm. Exotoxin Y (ExoY) is one such toxin. ExoY is a cyclase that produces …
Virulence is due to a type III secretion system (T3SS) that introduces exotoxins into the host
cell cytoplasm. Exotoxin Y (ExoY) is one such toxin. ExoY is a cyclase that produces …
Association of mortality with type III secretory protein phenotype in lower respiratory tract and systemic Pseudomonas aeruginosa infections My Citations
A Roy-Burman, RH Savel, S Racine, J Fujimoto… – CRITICAL CARE …, 2000
Association of mortality with type III secretory protein phenotype in lower respiratory
tract and systemic Pseudomonas aeruginosa infections. A Roy-Burman, RH Savel,
S Racine, J Fujimoto, NS Revadigar, T Sawa, D Frank, JP …
tract and systemic Pseudomonas aeruginosa infections. A Roy-Burman, RH Savel,
S Racine, J Fujimoto, NS Revadigar, T Sawa, D Frank, JP …
Feel the Burn, then Feel the Death. ExoU as a Phospholipase My Citations
E Bord, Z Farrell, W Heur, W Keslin, R Laughlin… – The FASEB Journal, 2013
A major cause of infection-related deaths in immunocompromised patients is the protein
toxin ExoU, encoded by the bacterium Pseudomonas aeruginosa. The Brown Deer SMART
(Students Modeling A Research Topic) Team has modeled ExoU using 3D printing …
toxin ExoU, encoded by the bacterium Pseudomonas aeruginosa. The Brown Deer SMART
(Students Modeling A Research Topic) Team has modeled ExoU using 3D printing …
Volume 61 January 1993 My Citations
SAB Jaynes, JC Newton, FM Enright, TG Snider III…
Isolation and Partial Characterization of Glycolipid Fractions from Mycobacterium avium Serovar
2 (Mycobacterium paratuberculosis 18) That Inhibit Activated Macrophages Major Stable Peptides
of Yersinia pestis Synthesized during the Low-Calcium Response Storage Reservoirs of …
2 (Mycobacterium paratuberculosis 18) That Inhibit Activated Macrophages Major Stable Peptides
of Yersinia pestis Synthesized during the Low-Calcium Response Storage Reservoirs of …
Pseudomonas aeruginosa induces the production and release of a cytotoxic factor from pulmonary endothelial cells My Citations
RD Balczon, A Morrow, B Edmonds, L Cauthen… – … BIOLOGY OF THE CELL, 2014
Pseudomonas aeruginosa induces the production and release of a cytotoxic factor from pulmonary
endothelial cells. RD Balczon, A Morrow, B Edmonds, L Cauthen, D Frank, T Stevens
MOLECULAR BIOLOGY OF THE CELL 25, AMER SOC CELL BIOLOGY, 12/2014.
endothelial cells. RD Balczon, A Morrow, B Edmonds, L Cauthen, D Frank, T Stevens
MOLECULAR BIOLOGY OF THE CELL 25, AMER SOC CELL BIOLOGY, 12/2014.
Infection of epithelial cells with Pseudomonas aeruginosa produces high concentrations of cCMP, cGMP and cUMP My Citations
U Voigt, DW Frank, V Kaever, R Seifert – NAUNYN-SCHMIEDEBERGS ARCHIVES …, 2011
Infection of epithelial cells with Pseudomonas aeruginosa produces high concentrations of
cCMP, cGMP and cUMP. U Voigt, DW Frank, V Kaever, R Seifert NAUNYN-SCHMIEDEBERGS
ARCHIVES OF PHARMACOLOGY 383, 16-16, SPRINGER, 3/2011.
cCMP, cGMP and cUMP. U Voigt, DW Frank, V Kaever, R Seifert NAUNYN-SCHMIEDEBERGS
ARCHIVES OF PHARMACOLOGY 383, 16-16, SPRINGER, 3/2011.
Pseudomonas aeruginosa nucleic acids encoding exoenzyme S activity and use thereof in detecting pseudomonas aeruginosa infection My Citations
JT Barbieri, DW Frank, SM Kulich – Biotechnology Advances, 1997
A genetic construct containing a coding region for exoenzyme S activity from Pseudomonas
aeruginosa is disclosed. An essentially pure protein preparation of the 49 kDa form of
exoenzyme S is also disclosed. The protein product of the genetic construct may be used …
aeruginosa is disclosed. An essentially pure protein preparation of the 49 kDa form of
exoenzyme S is also disclosed. The protein product of the genetic construct may be used …
The Pseudomonas AerugINOSa Exotoxin Y Induces Aggregated Endothelial Tau Release As A Putative Mechanism Of Cell Growth Suppression And Impaired … My Citations
TC Stevens, CD Ochoa, DW Frank, R Balczon… – Am J Respir Crit Care Med, 2013
Rationale: Exotoxin Y (ExoY) is a Pseudomonas aeruginosa toxin that is introduced into host
cells through the type 3 secretion system. Once inside the host cell cytoplasm ExoY
generates cyclic nucleotides that promote tau hyperphosphorylation and insolubility, …
cells through the type 3 secretion system. Once inside the host cell cytoplasm ExoY
generates cyclic nucleotides that promote tau hyperphosphorylation and insolubility, …
Pseudomonas aeruginosa toxin ExoY, a cUMP/cGMP-producing nucleotidyl cyclase My Citations
C Wölfel, DW Frank, S Wolter, V Kaever, R Seifert – NAUNYN-SCHMIEDEBERGS …, 2010
Pseudomonas aeruginosa toxin ExoY, a cUMP/cGMP-producing nucleotidyl cyclase.
C Woelfel, DW Frank, S Wolter, V Kaever, R Seifert NAUNYN-SCHMIEDEBERGS
ARCHIVES OF PHARMACOLOGY 381, 29-29, SPRINGER, 3/2010.
C Woelfel, DW Frank, S Wolter, V Kaever, R Seifert NAUNYN-SCHMIEDEBERGS
ARCHIVES OF PHARMACOLOGY 381, 29-29, SPRINGER, 3/2010.
The Pseudomonas Aeruginosa Exotoxin Y Impairs Microtubule Assembly In Pulmonary Microvascular Endothelial Eells My Citations
N Prasain, J Prater, M Alexeyev, B Zhu, S Sayner… – Am J Respir Crit Care Med, 2011
Rationale: Pseudomonas aeruginosa is an opportunistic pathogen that causes acute and
chronic pulmonary infections. During pulmonary infection, Pseudomonas uses a type III
secretion system (T3SS) to transfer an adenylyl cyclase termed ExoY to the cytoplasm of …
chronic pulmonary infections. During pulmonary infection, Pseudomonas uses a type III
secretion system (T3SS) to transfer an adenylyl cyclase termed ExoY to the cytoplasm of …
The Pseudomonas AerugINOSa Exotoxin Y Impairs Endothelial Cell Proliferation, Angiogenesis, And Vascular Repair Following Lung Injury My Citations
Rationale: ExoY is a Pseudomonas aeruginosa toxin that is introduced into host cells
through the type 3 secretion system (T3SS). Once inside the host cell cytoplasm ExoY
generates cyclic nucleotides that cause tau phosphorylation and microtubule breakdown. …
through the type 3 secretion system (T3SS). Once inside the host cell cytoplasm ExoY
generates cyclic nucleotides that cause tau phosphorylation and microtubule breakdown. …
The effector protein ExoY secreted by Pseudomonas aeruginosa augments the inflammatory reaction in the respiratory tract of mice My Citations
J Rothschuh, A Munder, B Tuemmler, D Frank… – NAUNYN- …, 2015
The effector protein ExoY secreted by Pseudomonas aeruginosa augments the
inflammatory reaction in the respiratory tract of mice. J Rothschuh, A Munder, B
Tuemmler, D Frank, R Seifert, C Hartwig NAUNYN-SCHMIEDEBERGS …
inflammatory reaction in the respiratory tract of mice. J Rothschuh, A Munder, B
Tuemmler, D Frank, R Seifert, C Hartwig NAUNYN-SCHMIEDEBERGS …
Pseudomonas aeruginosa infection causes endothelial cell Tau aggregation: evidence for an endothelial tauopathy (1176.5) My Citations
K Morrow, C Ochoa, M Alexeyev, D Frank, R Balczon… – The FASEB Journal, 2014
Abstract Our laboratory recently demonstrated that Pseudomonas aeruginosa exoenzyme Y
(ExoY) stimulates Tau hyperphosphorylation and insolubility-hallmarks of a “Tauopathy”.
Unlike neurodegenerative Tauopathies, we did not observe formation of Tau aggregates …
(ExoY) stimulates Tau hyperphosphorylation and insolubility-hallmarks of a “Tauopathy”.
Unlike neurodegenerative Tauopathies, we did not observe formation of Tau aggregates …
Type III secretory proteins in Pseudomonas aeruginosa. My Citations
H Sato, DW Frank, KA Brogden, FC Minion, N Cornick… – Virulence mechanisms of …, 2006
Abstract This chapter discusses virulence mechanisms in the opportunist bacteria
Pseudomonas aeruginosa, with emphasis on its type III secretory system (T3SS) and its
effector proteins. Relevant biochemical signalling cascades and host-pathogen …
Pseudomonas aeruginosa, with emphasis on its type III secretory system (T3SS) and its
effector proteins. Relevant biochemical signalling cascades and host-pathogen …
Pseudomonas Aeruginosa Exotoxin Y Increases Intracellular Levels Of Both CAMP And CGMP In Pulmonary Microvascular Endothelial Cells My Citations
M Alexeyev, D Frank, T Stevens, C Ochoa – Am J Respir Crit Care Med, 2011
Rationale: Pseudomonas aeruginosa is a gram-negative bacterium that causes
bronchopneumonia and acute lung injury in susceptible patients. P. aeruginosa acquires
virulence by upregulating a type III secretion system that is used to intoxicate host cells …
bronchopneumonia and acute lung injury in susceptible patients. P. aeruginosa acquires
virulence by upregulating a type III secretion system that is used to intoxicate host cells …
The Pseudomonas Aeruginosa Exotoxin Y Impairs Endothelial Cell Mobility My Citations
Rationale: Virulent Pseudomonas aeruginosa strains possess a functional type III secretion
system that is used to inject exotoxins into host cells. Exotoxin Y (ExoY) is one such toxin.
ExoY is a soluble adenylyl cyclase that, upon binding a mammalian cofactor, generates …
system that is used to inject exotoxins into host cells. Exotoxin Y (ExoY) is one such toxin.
ExoY is a soluble adenylyl cyclase that, upon binding a mammalian cofactor, generates …
Pseudomonas AerugINOSa Impairs The Activation Of Store Operated Calcium Entry Via Microtubule-Associated Protein 6 My Citations
CD Ochoa, TC Stevens, M Alexeyev, DW Frank… – Am J Respir Crit Care Med, 2012
Milwaukee, WI causes bronchopneumonia that can progress to acute lung injury. interacts
with and disrupts the Pseudomonas aeruginosa P. aeruginosa lung capillary endothelial cell
barrier resulting in alveolar edema and hemorrhage. uses a type III secretion system ( …
with and disrupts the Pseudomonas aeruginosa P. aeruginosa lung capillary endothelial cell
barrier resulting in alveolar edema and hemorrhage. uses a type III secretion system ( …
The effector protein ExoY secreted by Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for GTP My Citations
U Voigt, H Burhenne, L Sonnow, C Wölfel, C Spangler… – BMC Pharmacology, 2011
Background Pseudomonas aeruginosa is an important opportunistic pathogen causing
serious pulmonary, urogenital and systemic infections. P. aeruginosa injects four effector
proteins into host cells via the type III secretion system. ExoY is one of these proteins.
serious pulmonary, urogenital and systemic infections. P. aeruginosa injects four effector
proteins into host cells via the type III secretion system. ExoY is one of these proteins.
ExoY from Pseudomonas aeruginosa, a preferential guanylyl and uridylyl cyclase (610.2) My Citations
R Seifert, U Beckert, D Frank – The FASEB Journal, 2014
Abstract ExoY from Pseudomonas aeruginosa is injected into host cells via the type III
secretion system (Yahr TL et al., Proc Natl Acad Sci USA 95: 13899 (1998)). ExoY is
structurally similar to CyaA from Bordetella pertussis and edema factor (EF) from Bacillus …
secretion system (Yahr TL et al., Proc Natl Acad Sci USA 95: 13899 (1998)). ExoY is
structurally similar to CyaA from Bordetella pertussis and edema factor (EF) from Bacillus …
Francisella tularensis Mutagenesis of My Citations
C Zahrt, DW Frank
MATERIALS AND METHODS Bacterial strains, plasmids, and growth conditions. All
bacterial strains and plasmids used in this study are listed in Table 1. F. tularensis LVS was
routinely grown at 37 C in modified Mueller-Hinton (MH) broth or on agar (Difco …
bacterial strains and plasmids used in this study are listed in Table 1. F. tularensis LVS was
routinely grown at 37 C in modified Mueller-Hinton (MH) broth or on agar (Difco …
P-aeruginosa adenylyl cyclase (ExoY) increases cAMP causing intercellular gap formation in pulmonary microvascular endothelium My Citations
S Sayner, D Frank, J VandeWaa, T Stevens – FASEB JOURNAL, 2003
P-aeruginosa adenylyl cyclase (ExoY) increases cAMP causing intercellular gap formation in
pulmonary microvascular endothelium. S Sayner, D Frank, J VandeWaa, T Stevens FASEB
JOURNAL 17:44, A412-A412, FEDERATION AMER SOC EXP BIOL, 3/2003.
pulmonary microvascular endothelium. S Sayner, D Frank, J VandeWaa, T Stevens FASEB
JOURNAL 17:44, A412-A412, FEDERATION AMER SOC EXP BIOL, 3/2003.
Pseudomonas aeruginosa Exoenzymes U and Y Induce a Transmissible Endothelial Tauopathy My Citations
K Morrow, C Ochoa, R Balczon, L Cauthen, M Alexeyev… – The FASEB Journal, 2015
Abstract Here, we tested the hypothesis that Pseudomonas aeruginosa type 3 secretion
system effectors ExoY and ExoU induce endothelial tau oligomerization and release,
causing transmissible cytotoxicity characteristic of an infectious tauopathy. Both the …
system effectors ExoY and ExoU induce endothelial tau oligomerization and release,
causing transmissible cytotoxicity characteristic of an infectious tauopathy. Both the …
Pseudomonas aeruginosa exoenzymes U and Y induce a transmissible endothelial proteinopathy My Citations
KA Morrow, CD Ochoa, R Balczon, C Zhou, L Cauthen… – American Journal of …, 2015
Abstract Here, we tested the hypothesis that Pseudomonas aeruginosa type 3 secretion
system effectors ExoY and ExoU induce release of a high molecular weight endothelial tau,
causing transmissible cell injury characteristic of an infectious proteinopathy. Both the …
system effectors ExoY and ExoU induce release of a high molecular weight endothelial tau,
causing transmissible cell injury characteristic of an infectious proteinopathy. Both the …
Functional association of endogenous adenylyl cyclases and phosphodiesterases in pulmonary microvascular endothelial cells revealed by P-aeruginosa adenylyl … My Citations
S Sayner, D Frank, J VandeWaa, T Stevens – FASEB JOURNAL, 2003
Functional association of endogenous adenylyl cyclases and phosphodiesterases
in pulmonary microvascular endothelial cells revealed by P-aeruginosa adenylyl
cyclase, ExoY. S Sayner, D Frank, J VandeWaa, T Stevens …
in pulmonary microvascular endothelial cells revealed by P-aeruginosa adenylyl
cyclase, ExoY. S Sayner, D Frank, J VandeWaa, T Stevens …
Pseudomonas AerugINOSa Decreases Merlin Phosphorylation In Pulmonary Endothelium As A Mechanism Of Growth Suppression My Citations
Rationale: Pulmonary microvascular endothelial cells (PMVECs) display a proproliferative
phenotype when compared to pulmonary artery endothelial cells (PAECs); mechanisms
responsible for this growth disparity are poorly understood. The cytoskeletal linker moesin …
phenotype when compared to pulmonary artery endothelial cells (PAECs); mechanisms
responsible for this growth disparity are poorly understood. The cytoskeletal linker moesin …
Pseudomonas AerugINOSa Exotoxin Promotes Microtubule-Associated Protein 6 Binding To Pulmonary Microvascular Endothelial Cell Microtubules My Citations
MJ Robson, TC Stevens, R Balczon, DW Frank… – Am J Respir Crit Care Med, 1862
Microtubules spontaneously disassemble upon exposure to temperatures below 15 C, a
phenomenon observed in cells o across all phyla. However, animals adapted to cold
environments express a microtubule-associated protein (MAP6, also known as stable …
phenomenon observed in cells o across all phyla. However, animals adapted to cold
environments express a microtubule-associated protein (MAP6, also known as stable …
Pseudomonas aeruginosa toxin ExoY-biochemical characterization of a nucleotidyl cyclaseMy Citations
L Sonnow, DW Frank, V Keever, J von der Ohe… – NAUNYN- …, 2011
Pseudomonas aeruginosa toxin ExoY-biochemical characterization of a nucleotidyl cyclase.
L Sonnow, DW Frank, V Keever, J von der Ohe, R Seifert NAUNYN-SCHMIEDEBERGS
ARCHIVES OF PHARMACOLOGY 383, 25-25, SPRINGER, 3/2011.
L Sonnow, DW Frank, V Keever, J von der Ohe, R Seifert NAUNYN-SCHMIEDEBERGS
ARCHIVES OF PHARMACOLOGY 383, 25-25, SPRINGER, 3/2011.
Heterogeneity of Pulmonary Endothelial Cyclic Nucleotide Response to Pseudomonas aeruginosa Infection My Citations
K Morrow, R Seifert, A Britain, C Ochoa, E Cioffi… – The FASEB Journal, 2015
Abstract Here, we tested the hypothesis that a promiscuous bacterial cyclase generates a
diverse cyclic nucleotide response in pulmonary endothelium. Pulmonary endothelial cells
were infected with a gram-negative bacterium Pseudomonas aeruginosa. A strain that …
diverse cyclic nucleotide response in pulmonary endothelium. Pulmonary endothelial cells
were infected with a gram-negative bacterium Pseudomonas aeruginosa. A strain that …
Red Phalaenopsis hybrids My Citations
J Martin, HP Norton, M Rose, CF Fighetti, DW Frank – American Orchid Society …, 1993
The Pseudomonas Aeruginosa Type Three Secretion System Inhibits Store Operated Calcium Entry My Citations
TC Stevens, C Ochoa, D Frank, T Stevens, M Robson – Am J Respir Crit Care Med, 1944
Rationale: Pseudomonas aeruginosa is a principal cause of pneumonia that can progress to
sepsis and acute lung injury. This bacterium interacts with endothelium, and utilizes a type III
secretion system (T3SS) to introduce exotoxins that evade immunodetection. Four toxins …
sepsis and acute lung injury. This bacterium interacts with endothelium, and utilizes a type III
secretion system (T3SS) to introduce exotoxins that evade immunodetection. Four toxins …
The Role of Exoenzyme S in Pseudomonas aeruginosa Infections My Citations
BH Inglewski, DW Frank, S Steinbach, TI Nicas… – 1988
Abstract: We utilized genetic and immunological methods to determine the role of
exoenzyme S in Pseudomonas aeruginosa infections. An exoenzyme S deficient mutant,
388 exsl: Tn1, was compared to its S (+) parent strain in the burned mouse model and …
exoenzyme S in Pseudomonas aeruginosa infections. An exoenzyme S deficient mutant,
388 exsl: Tn1, was compared to its S (+) parent strain in the burned mouse model and …
Pseudomonas aeruginosa induced cytotoxicity for corneal epithelium: Cell invasion is not a prerequisite. My Citations
SMJ Fleiszig, DJ Evans, V Vallas, DW Frank – … OPHTHALMOLOGY & VISUAL …, 1997
Pseudomonas aeruginosa induced cytotoxicity for corneal epithelium: Cell invasion is not a
prerequisite. SMJ Fleiszig, DJ Evans, V Vallas, DW Frank INVESTIGATIVE OPHTHALMOLOGY
& VISUAL SCIENCE 38:44, 5334-5334, LIPPINCOTT-RAVEN PUBL, 3/1997.
prerequisite. SMJ Fleiszig, DJ Evans, V Vallas, DW Frank INVESTIGATIVE OPHTHALMOLOGY
& VISUAL SCIENCE 38:44, 5334-5334, LIPPINCOTT-RAVEN PUBL, 3/1997.
P. aeruginosa ExoY Increases Lung Endothelial Permeability with a Concomitant Decrease in Lung Vascular Compliance My Citations
N Prasain, DF Alvarez, DW Frank, T Stevens – The FASEB Journal, 2009
ABSTRACT The P. aeruginosa toxin, ExoY, when injected through a type III secretion system
(TTSS) into endothelial cells, produces a cytosolic cAMP pool and induces endothelial cell
retraction. However, it is unclear whether ExoY-induced endothelial cell retraction …
(TTSS) into endothelial cells, produces a cytosolic cAMP pool and induces endothelial cell
retraction. However, it is unclear whether ExoY-induced endothelial cell retraction …
Pseudomonas aeruginosa toxins My Citations
S Rolsma, DW Frank, JT Barbieri – The Comprehensive Sourcebook of Bacterial …, 2015
Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that grows in diverse
environments, including soil and water reservoirs, the mammalian lung, and other sites of
infection. P. aeruginosa is an opportunistic pathogen that utilizes surface and secreted …
environments, including soil and water reservoirs, the mammalian lung, and other sites of
infection. P. aeruginosa is an opportunistic pathogen that utilizes surface and secreted …
The Pseudomonas Aeruginosa Exotoxin U Causes Endothelial Cell Tau Aggregation: Evidence For A Tauopathy Of The Lung My Citations
CD Ochoa, M Alexeyev, DW Frank, JP Audia… – Am J Respir Crit Care Med, 2013
Results: Doxycycline-induced ExoU causedPMVEC gap formation in a dose-dependent
manner. At low doxycycline concentrations (0.01 µg/mL), intercellular gaps formed within 2
hours and progressively increased in size up to 8 hours. Gap formation was paralleled by …
manner. At low doxycycline concentrations (0.01 µg/mL), intercellular gaps formed within 2
hours and progressively increased in size up to 8 hours. Gap formation was paralleled by …
Molecular Studies of Pseudomonas aeruginasa Exotoxin A My Citations
DW FRANK, E SUSAN, H Wesr – Molecular Basis of Bacterial Pathogenesis, 1990
Exotoxin A is the most toxic of the numerous extracellular proteins (ex-oenzyme S, elastase,
alkaline protease, phospholipase C, and leukocidin) pro-duced by Pseudomonas
aeruginasa. As an opportunistic. pathogen, this organism infects injured, immunodeficient, …
alkaline protease, phospholipase C, and leukocidin) pro-duced by Pseudomonas
aeruginasa. As an opportunistic. pathogen, this organism infects injured, immunodeficient, …
A method of inhibiting, moderating or diagnosing pseudomonas aeruginosa infection is disclosed. My Citations
DW Frank, TL Yahr, T Sawa, J Wiener-Kronish – US Patent 6,309,651, 2001
A method of inhibiting, moderating or diagnosing Pseudomonas aeruginosa infection is
disclosed. In one embodiment, this method comprises inoculating a patient with an effective amount
of PcrV antigen….http://www.google.com/patents/US6309651?utm_source=gb-gplus- …
disclosed. In one embodiment, this method comprises inoculating a patient with an effective amount
of PcrV antigen….http://www.google.com/patents/US6309651?utm_source=gb-gplus- …
Art-shade phalaenopsis. My Citations
M Rose, HP Norton, J Martin, CF Fighetti, DW Frank – American Orchid Society …, 1993
Cross Kingdom Activators of Five Classes of Bacterial Effectors My Citations
DM Anderson, JB Feix, DW Frank – PLoS Pathog, 2015
Copyright:© 2015 Anderson et al. This is an open access article distributed under the terms
of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited
of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited
ASSOCIATION OF PSEUDOMONAS-AERUGINOSA CYTOTOXICITY WITH THE PRODUCTION OF EXOENZYME-S My Citations
JP WIENERKRONISH, G Apodaca, DW FRANK… – FASEB JOURNAL, 1993
ASSOCIATION OF PSEUDOMONAS-AERUGINOSA CYTOTOXICITY WITH THE PRODUCTION
OF EXOENZYME-S. JP WIENERKRONISH, G APODACA, DW FRANK, K MOSTOV FASEB
JOURNAL 7:44, A675-A675, FEDERATION AMER SOC EXP BIOL, 2/1993.
OF EXOENZYME-S. JP WIENERKRONISH, G APODACA, DW FRANK, K MOSTOV FASEB
JOURNAL 7:44, A675-A675, FEDERATION AMER SOC EXP BIOL, 2/1993.
AraC Family Regulators and Transcriptional Control of Bacterial Virulence Determinants My Citations
DW Frank, ML Hunt – 2003
This chapter focuses on selected members of the AraC/XylS family of regulatory proteins as
a potential model system to study the induction of genes related to bacterial virulence in
human infections. The structural analysis of an AraC family member, Rob, demonstrated …
a potential model system to study the induction of genes related to bacterial virulence in
human infections. The structural analysis of an AraC family member, Rob, demonstrated …
In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins My Citations
SL Rolsma, DW Frank – Pseudomonas Methods and Protocols, 2014
Abstract Pseudomonas aeruginosa secretes numerous toxins and destructive enzymes that
play distinct roles in pathogenesis. The Type III secretion system (T3SS) of Pseudomonas is
a system that delivers a subset of toxins directly into the cytoplasm of eukaryotic cells. The …
play distinct roles in pathogenesis. The Type III secretion system (T3SS) of Pseudomonas is
a system that delivers a subset of toxins directly into the cytoplasm of eukaryotic cells. The …
Pseudomonas aeruginosa inhibits Fc receptor-mediated phagocytosis. My Citations
M Ohara, T Sawa, K Kurahashi, TL Yahr, DW Frank… – JOURNAL OF …, 1998
Pseudomonas aeruginosa inhibits Fc receptor-mediated phagocytosis. M Ohara, T Sawa,
K Kurahashi, TL Yahr, DW Frank, JP Wiener-Kronish, MA Gropper JOURNAL OF
INVESTIGATIVE MEDICINE 46:33, 218A-218A, SLACK INC, 3/1998.
K Kurahashi, TL Yahr, DW Frank, JP Wiener-Kronish, MA Gropper JOURNAL OF
INVESTIGATIVE MEDICINE 46:33, 218A-218A, SLACK INC, 3/1998.
Targets Of Subplasma Membrane Versus Cytosolic Adenylyl Cyclase Activity Demonstrate The Bidirectional Nature Of The CAMP Diffusion Barricade That Maintains … My Citations
RATIONALE: Strongly adherent pulmonary microvascular endothelial cells (PMVECs)
interact to form the pulmonary capillary barrier, necessary to maintain efficient gas exchange
in the lung. Transmembrane adenylyl cyclase activity generates cAMP restricted to the …
interact to form the pulmonary capillary barrier, necessary to maintain efficient gas exchange
in the lung. Transmembrane adenylyl cyclase activity generates cAMP restricted to the …
Reproducible and Quantitative Model of Infection of Dermacentor variabilis with the Live Vaccine Strain of Francisella tularensis My Citations
J Coburn, T Maier, M Casey, L Padmore, H Sato… – Applied and environmental …, 2015
ABSTRACT Pathogen life cycles in mammalian hosts have been studied extensively, but
studies with arthropod vectors represent considerable challenges. In part this is due to the
difficulty of delivering a reproducible dose of bacteria to follow arthropod-associated …
studies with arthropod vectors represent considerable challenges. In part this is due to the
difficulty of delivering a reproducible dose of bacteria to follow arthropod-associated …
EXOENZYME-S SECRETED BY PSEUDOMONAS-AERUGINOSA CAUSES ALVEOLAR EPITHELIAL DAMAGE IN A RABBIT MODEL OF PNEUMONIA My Citations
I Kudoh, JF Pittet, D Frank, T Sakuma, MA Matthay… – FASEB JOURNAL, 1992
EXOENZYME-S SECRETED BY PSEUDOMONAS-AERUGINOSA CAUSES ALVEOLAR
EPITHELIAL DAMAGE IN A RABBIT MODEL OF PNEUMONIA. I KUDOH, JF PITTET,
D FRANK, T SAKUMA, MA MATTHAY, J WIENERKRONISH …
EPITHELIAL DAMAGE IN A RABBIT MODEL OF PNEUMONIA. I KUDOH, JF PITTET,
D FRANK, T SAKUMA, MA MATTHAY, J WIENERKRONISH …
Rapid IL-10 upregulation in lung in cytotoxic Pseudomonas aeruginosa pneumonia My Citations
T Sawa, M Ohara, K Kurahashi, MA Gropper, DW Frank… – FASEB JOURNAL, 1998
Rapid IL-10 upregulation in lung in cytotoxic Pseudomonas aeruginosa pneumonia. T Sawa,
M Ohara, K Kurahashi, MA Gropper, DW Frank, JP Weiner-Kronish FASEB JOURNAL
12:55, A1071-A1071, FEDERATION AMER SOC EXP BIOL, 3/1998.
M Ohara, K Kurahashi, MA Gropper, DW Frank, JP Weiner-Kronish FASEB JOURNAL
12:55, A1071-A1071, FEDERATION AMER SOC EXP BIOL, 3/1998.
Yellow Phalaenopsis My Citations
CF Fighetti, DW Frank, J Martin, HP Norton, M Rose – American Orchid Society …, 1993
Pseudomonas aeruginosa ExoY, a cyclic GMP-and cyclic UMP-generating nucleotidyl cyclase My Citations
R Seifert, C Hartwig, S Wolter, D Reinecke… – BMC Pharmacology and …, 2013
Background In previous studies we showed that in addition to the cyclic nucleotides
(cNMPs) cAMP and cGMP, mammalian cells also contain cCMP and cUMP [for review see
[1],[2]]. cCMP and cUMP are generated by the bacterial exotoxins CyaA from Bordetella …
(cNMPs) cAMP and cGMP, mammalian cells also contain cCMP and cUMP [for review see
[1],[2]]. cCMP and cUMP are generated by the bacterial exotoxins CyaA from Bordetella …
Heterogeneity of pulmonary endothelial cyclic nucleotide response to Pseudomonas aeruginosa ExoY infection My Citations
KA Morrow, R Seifert, V Kaever, AL Britain, SL Sayner… – American Journal of …, 2015
Abstract Here, we tested the hypothesis that a promiscuous bacterial cyclase synthesizes
purine and pyrimidine cyclic nucleotides in the pulmonary endothelium. To test this
hypothesis, pulmonary endothelial cells were infected with a strain of the Gram-negative …
purine and pyrimidine cyclic nucleotides in the pulmonary endothelium. To test this
hypothesis, pulmonary endothelial cells were infected with a strain of the Gram-negative …
Type III secretory proteins in Pseudomonas aeruginosa My Citations
H Sato, DW Frank – Virulence Mechanisms of Bacterial Pathogens, 2007
Pseudomonas aeruginosa is a normal inhabitant of soil and water and is ubiquitous in the
environment. It possesses a large genome encoding diverse arrays of metabolic, catabolic,
and virulence-related proteins and regulatory systems that account for its seemingly …
environment. It possesses a large genome encoding diverse arrays of metabolic, catabolic,
and virulence-related proteins and regulatory systems that account for its seemingly …
Pathogenesis of acute lung injury caused by Pseudomonas aeruginosa-Major contribution of type III secretory system. My Citations
T Sawa, M Ohara, K Kurahashi, TL Yahr, MA Gropper… – JOURNAL OF …, 1998
Pathogenesis of acute lung injury caused by Pseudomonas aeruginosa-Major
contribution of type III secretory system. T Sawa, M Ohara, K Kurahashi, TL Yahr, MA
Gropper, DW Frank, JP Wiener-Kronish JOURNAL OF INVESTIGATIVE …
contribution of type III secretory system. T Sawa, M Ohara, K Kurahashi, TL Yahr, MA
Gropper, DW Frank, JP Wiener-Kronish JOURNAL OF INVESTIGATIVE …
Humanized anti-PerV monoclonal antibody IM166 binds to the type III toxin delivery system of Pseudomonas aeruginosa and prevents mortality in an animal model of … My Citations
EG Spack, T Sawa, J Wiener-Kronish, DW Frank… – FASEB JOURNAL, 2002
Humanized anti-PerV monoclonal antibody IM166 binds to the type III toxin delivery
system of Pseudomonas aeruginosa and prevents mortality in an animal model
of Pseudomonas-induced pneumonia. EG Spack, T Sawa, J …
system of Pseudomonas aeruginosa and prevents mortality in an animal model
of Pseudomonas-induced pneumonia. EG Spack, T Sawa, J …
ExoY increases Pseudomonas aeruginosa virulence My Citations
N Prasain, DF Alvarez, DW Frank, T Stevens – The FASEB Journal, 2008
ABSTRACT Pseudomonas aeruginosa is a principal cause of pneumonia that progresses to
sepsis and acute lung injury. Bacterial virulence is due to the type III secretion system
(TTSS), which is a functional bacterial needle that introduces exotoxins into host cells. …
sepsis and acute lung injury. Bacterial virulence is due to the type III secretion system
(TTSS), which is a functional bacterial needle that introduces exotoxins into host cells. …
Method of and compositions for immunization with the pseudomonas V antigen My Citations
DW Frank, J Wiener-Kronish, TL Yahr, T Sawa, RB Fritz – US Patent 6,827,935, 2004
Screen reader users: click this link for accessible mode. Accessible mode has the same essential
features but works better with your reader. … A method of inhibiting, moderating or diagnosing
Pseudomonas aeruginosa infection is disclosed. In one embodiment, this method …
features but works better with your reader. … A method of inhibiting, moderating or diagnosing
Pseudomonas aeruginosa infection is disclosed. In one embodiment, this method …
Method and compositions for immunization with the Pseudomonas V antigen My Citations
DW Frank, J Wiener-Kronish, TL Yahr, T Sawa, RB Fritz – US Patent 8,101,347, 2012
1. An isolated monoclonal antibody that specifically binds to Pseudomonas aeruginosa PcrV
antigen, wherein the antibody comprises a heavy chain (SEQ. ID NO: 2) and a light chain
(SEQ. ID NO: 4) wherein the antibody or a fragment thereof specifically binds to an …
antigen, wherein the antibody comprises a heavy chain (SEQ. ID NO: 2) and a light chain
(SEQ. ID NO: 4) wherein the antibody or a fragment thereof specifically binds to an …
Evidence that all Pseudomonas aeruginosa strains may be inherently capable of invading corneal epithelial cells and that cytotoxicity is regulated by EXSA. My Citations
SMJ Fleiszig, JP WienerKronish, V Vallas, KE Mostov… – … & VISUAL SCIENCE, 1996
Evidence that all Pseudomonas aeruginosa strains may be inherently capable of invading
corneal epithelial cells and that cytotoxicity is regulated by EXSA. SMJ Fleiszig, JP
WienerKronish, V Vallas, KE Mostov, DW Frank INVESTIGATIVE …
corneal epithelial cells and that cytotoxicity is regulated by EXSA. SMJ Fleiszig, JP
WienerKronish, V Vallas, KE Mostov, DW Frank INVESTIGATIVE …
Pseudomonas aeruginosa toxins My Citations
AW Maresso, DW Frank, JT Barbieri – The Comprehensive Sourcebook of Bacterial …, 2005
Bacterial pathogens subvert host responses through multiple and diverse mechanisms,
while eukaryotic organisms utilize innate and acquired immunity to prevent bacterial
pathogens from establishing infections. A successful pathogen must initially permeate a …
while eukaryotic organisms utilize innate and acquired immunity to prevent bacterial
pathogens from establishing infections. A successful pathogen must initially permeate a …
Ubiquitin activates patatin-like phospholipases from multiple bacterial species My Citations
DM Anderson, H Sato, AT Dirck, JB Feix, DW Frank – Journal of bacteriology, 2015
ABSTRACT Phospholipase A 2 enzymes are ubiquitously distributed throughout the
prokaryotic and eukaryotic kingdoms and are utilized in a wide array of cellular processes
and physiological and immunological responses. Several patatin-like phospholipase …
prokaryotic and eukaryotic kingdoms and are utilized in a wide array of cellular processes
and physiological and immunological responses. Several patatin-like phospholipase …
Pseudomonas aeruginosa exoenzyme S, a bifunctional cytotoxin secreted by a type-III pathway My Citations
JT Barbieri, DW Frank – Bacterial Protein Toxins, 2000
Abstract Cystic-fibrosis patients, burn victims, and immunocompromised individuals are
particularly susceptible to infection by Pseudomonas aeruginosa, a Gram-negative
opportunistic pathogen.(Bodey et al. 1983; Roilides et al. 1992; Mendelson et al. 1994). …
particularly susceptible to infection by Pseudomonas aeruginosa, a Gram-negative
opportunistic pathogen.(Bodey et al. 1983; Roilides et al. 1992; Mendelson et al. 1994). …
Cell surface antigens of Bordetella pertussis. My Citations
CD Parker, LV Branes, SK Armstrong, DW Frank… – Developments in biological …, 1984
Cell surface-specific radiolabelling techniques, monoclonal antibody analyses, and
chemical and physical isolation of cell fractions were used to study the surface of B.
pertussis cells. Four surface specific proteins were identified by radioiodination and …
chemical and physical isolation of cell fractions were used to study the surface of B.
pertussis cells. Four surface specific proteins were identified by radioiodination and …
In the absence of effector proteins, the Pseudomonas aeruginosa type three secretion system needle tip complex contributes to lung injury and systemic inflammatory … My Citations
JP Audia, AS Lindsey, NA Housley, CR Ochoa, C Zhou… – PloS one, 2013
Abstract Herein we describe a pathogenic role for the Pseudomonas aeruginosa type three
secretion system (T3SS) needle tip complex protein, PcrV, in causing lung endothelial injury.
We first established a model in which P. aeruginosa wild type strain PA103 caused …
secretion system (T3SS) needle tip complex protein, PcrV, in causing lung endothelial injury.
We first established a model in which P. aeruginosa wild type strain PA103 caused …
Control of bacterial soft rot caused by Pseudomonas cattleya in the culture of PhalaenopsisMy Citations
DW Frank – The Orchid digest (USA), 1988
[40] Regulation of expression of Pseudomonas exotoxin a by iron My Citations
DW Frank, DG Storey – Methods in enzymology, 1994
ScienceDirect is phasing out support for older versions of Internet Explorer on Jan
12, 2016. For the best product experience, we recommend you upgrade to a newer
version of IE or use a different browser: Firefox or Chrome. …
12, 2016. For the best product experience, we recommend you upgrade to a newer
version of IE or use a different browser: Firefox or Chrome. …
Genetic studies of Pseudomonas aeruginosa toxin A. My Citations
DW Frank, SF Steinbach, JM Bradley, BH Iglewski – … BAKTERIOL. MIKROBIOL. HYG …, 1986
A number of toxin A mutants have been isolated and characterized, the toxin A structural
gene has been cloned and sequenced and a gene which positively regulates toxin A has
also been cloned. The authors have used these mutants and cloned genes to determine …
gene has been cloned and sequenced and a gene which positively regulates toxin A has
also been cloned. The authors have used these mutants and cloned genes to determine …
Surface antigens of Bordetella pertussis My Citations
CD Parker, SK Armstrong, DW Frank – Immunobiology of Proteins and Peptides—III, 1985
Abstract Bordetella pertussis and other Bordetella species cause respiratory infections in
humans and in a variety of animals. Clinical isolates of B. pertussis have multiple virulence
factors, several of which have been reported to induce protective immunity. Using cell …
humans and in a variety of animals. Clinical isolates of B. pertussis have multiple virulence
factors, several of which have been reported to induce protective immunity. Using cell …
Induced conformational changes in the activation of the Pseudomonas aeruginosa type III toxin, ExoU My Citations
MA Benson, SM Komas, KM Schmalzer, MS Casey… – Biophysical journal, 2011
ExoU is a 74-kDa, water-soluble toxin injected directly into mammalian cells through the
type III secretion system of the opportunistic pathogen, Pseudomonas aeruginosa. Previous
studies have shown that ExoU is a Ca2+-independent phospholipase that requires a …
type III secretion system of the opportunistic pathogen, Pseudomonas aeruginosa. Previous
studies have shown that ExoU is a Ca2+-independent phospholipase that requires a …
Research topic on Pseudomonas aeruginosa, biology, genetics, and host–pathogen interactions My Citations
DW Frank – … Aeruginosa, Biology, Genetics, and Host-pathogen …, 2012
Over the years, Pseudomonas aeruginosa has served as a paradigm for the study of gene
expression, metabolism, and pathogenesis. The large genome, which approaches the
complexity and size of lower eukaryotes, and an abundance of regulators, facilitates …
expression, metabolism, and pathogenesis. The large genome, which approaches the
complexity and size of lower eukaryotes, and an abundance of regulators, facilitates …
Intoxication of Host Cells by the T3SS Phospholipase ExoU: PI (4, 5) P 2-Associated, Cytoskeletal Collapse and Late Phase Membrane Blebbing My Citations
H Sato, DW Frank – PloS one, 2014
Abstract Pseudomonas aeruginosa is an opportunistic pathogen that is associated with
hospital-acquired infections, ventilator-associated pneumonia, and morbidity of
immunocompromised individuals. A subpopulation of P. aeruginosa encodes a protein, …
hospital-acquired infections, ventilator-associated pneumonia, and morbidity of
immunocompromised individuals. A subpopulation of P. aeruginosa encodes a protein, …
Pseudomonas aeruginosa exotoxin Y-mediated tau hyperphosphorylation impairs microtubule assembly in pulmonary microvascular endothelial cells My Citations
R Balczon, N Prasain, C Ochoa, J Prater, B Zhu… – PloS one, 2013
Abstract Pseudomonas aeruginosa uses a type III secretion system to introduce the adenylyl
and guanylyl cyclase exotoxin Y (ExoY) into the cytoplasm of endothelial cells. ExoY
induces Tau hyperphosphorylation and insolubility, microtubule breakdown, barrier …
and guanylyl cyclase exotoxin Y (ExoY) into the cytoplasm of endothelial cells. ExoY
induces Tau hyperphosphorylation and insolubility, microtubule breakdown, barrier …
cCMP and cUMP occur in vivo My Citations
H Bähre, C Hartwig, A Munder, S Wolter, T Stelzer… – … and biophysical research …, 2015
Abstract Mammalian cells contain the cyclic pyrimidine nucleotides cCMP and cUMP. It is
unknown whether these tentative new second messenger molecules occur in vivo. We used
high performance liquid chromatography quadrupole tandem mass spectrometry to …
unknown whether these tentative new second messenger molecules occur in vivo. We used
high performance liquid chromatography quadrupole tandem mass spectrometry to …
Isolation and characterization of an attenuated strain of Pseudomonas aeruginosa AC869, a 3, 5-dichlorobenzoate degrader. My Citations
X Zhou, SE George, DW Frank, M Utley, I Gilmour… – Applied and environmental …, 1997
ABSTRACT Pseudomonas aeruginosa AC869, a 3, 5-dichlorobenzoate degrader, is a
mouse pathogen and has a reported 50% lethal dose (LD50) of 1.05 x 10 (7) CFU when
given intranasally to C3H/HeJ mice (SE George, MJ Kohan, MI Gilmour, MS Taylor, HG …
mouse pathogen and has a reported 50% lethal dose (LD50) of 1.05 x 10 (7) CFU when
given intranasally to C3H/HeJ mice (SE George, MJ Kohan, MI Gilmour, MS Taylor, HG …
A sensitive fluorescence-based assay for the detection of ExoU-mediated PLA 2 activity My Citations
MA Benson, KM Schmalzer, DW Frank – Clinica Chimica Acta, 2010
BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen that causes
disease in immunocompromised individuals, burn victims, and cystic fibrosis patients.
Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can …
disease in immunocompromised individuals, burn victims, and cystic fibrosis patients.
Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can …
Pseudomonas aeruginosa nucleic acids encoding exoenzyme S activity and use thereof in detecting pseudomonas aeruginosa infection My Citations
JT Barbieri, DW Frank, SM Kulich – US Patent 5,599,665, 1997
A genetic construct containing a coding region for exoenzyme S activity from Pseudomonas
aeruginosa is disclosed. A essentially pure protein preparation of the 49 kDa form of
exoenzyme S is also disclosed. The protein product of the genetic construct may be used …
aeruginosa is disclosed. A essentially pure protein preparation of the 49 kDa form of
exoenzyme S is also disclosed. The protein product of the genetic construct may be used …
Mechanisms of lung epithelial cell injury by Pseudomonas aeruginosa My Citations
JP Wiener-Kronish, D Frank, T Sawa – Molecular biology of acute lung injury, 2001
Abstract The interactions between hosts and pathogens have evolved to select for gene
combinations that can confer an advantage to either combatant. Bacteria have evolved
several methods of inducing cell injury and are an important cause of acute lung injury ( …
combinations that can confer an advantage to either combatant. Bacteria have evolved
several methods of inducing cell injury and are an important cause of acute lung injury ( …
Identification of the major ubiquitin-binding domain of the Pseudomonas aeruginosa ExoU A2 phospholipase My Citations
DM Anderson, JB Feix, AL Monroe, FC Peterson… – Journal of Biological …, 2013
↵1 To whom correspondence should be addressed: Dept. of Microbiology and Molecular Genetics
and the Center for Infectious Disease Research, Medical College of Wisconsin, 8701 Watertown
Plank Rd., Milwaukee, WI 53226. Tel.: 414-955-8766; Fax: 414-955-6535; E-mail: …
and the Center for Infectious Disease Research, Medical College of Wisconsin, 8701 Watertown
Plank Rd., Milwaukee, WI 53226. Tel.: 414-955-8766; Fax: 414-955-6535; E-mail: …
Modified needle-tip PcrV proteins reveal distinct phenotypes relevant to the control of type III secretion and intoxication by Pseudomonas aeruginosa My Citations
H Sato, ML Hunt, JJ Weiner, AT Hansen, DW Frank – PLoS One, 2011
Abstract The type III secretion system (T3SS) is employed to deliver effector proteins to the
cytosol of eukaryotic hosts by multiple species of Gram-negative bacteria, including
Pseudomonas aeruginosa. Translocation of effectors is dependent on the proteins …
cytosol of eukaryotic hosts by multiple species of Gram-negative bacteria, including
Pseudomonas aeruginosa. Translocation of effectors is dependent on the proteins …
Activation of ExoU phospholipase activity requires specific C-terminal regions My Citations
KM Schmalzer, MA Benson, DW Frank – Journal of bacteriology, 2010
ABSTRACT Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that
utilizes a type III secretion system to subvert host innate immunity. Of the 4 known effector
proteins injected into eukaryotic cells, ExoS and ExoU are cytotoxic. The cytotoxic …
utilizes a type III secretion system to subvert host innate immunity. Of the 4 known effector
proteins injected into eukaryotic cells, ExoS and ExoU are cytotoxic. The cytotoxic …
The Pseudomonas aeruginosa exoenzyme Y impairs endothelial cell proliferation and vascular repair following lung injury My Citations
TC Stevens, CD Ochoa, KA Morrow, MJ Robson… – American Journal of …, 2014
Abstract Exoenzyme Y (ExoY) is a Pseudomonas aeruginosa toxin that is introduced into
host cells through the type 3 secretion system (T3SS). Once inside the host cell cytoplasm,
ExoY generates cyclic nucleotides that cause tau phosphorylation and microtubule …
host cells through the type 3 secretion system (T3SS). Once inside the host cell cytoplasm,
ExoY generates cyclic nucleotides that cause tau phosphorylation and microtubule …
Five mechanisms of manipulation by bacterial effectors: a ubiquitous theme My Citations
DM Anderson, DW Frank – PLoS Pathog, 2012
Copyright:© Anderson, Frank. This is an open-access article distributed under the terms of
the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original author and source are credited.
the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original author and source are credited.
ExoY from Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for cGMP and cUMP formation My Citations
U Beckert, S Wolter, C Hartwig, H Bähre, V Kaever… – … and biophysical research …, 2014
Abstract In addition to the well known second messengers cAMP and cGMP, mammalian
cells contain the cyclic pyrimidine nucleotides cCMP and cUMP. Soluble guanylyl cyclase
and soluble adenylyl cyclase produce all four cNMPs. Several bacterial toxins exploit …
cells contain the cyclic pyrimidine nucleotides cCMP and cUMP. Soluble guanylyl cyclase
and soluble adenylyl cyclase produce all four cNMPs. Several bacterial toxins exploit …
Filamin A is a phosphorylation target of membrane but not cytosolic adenylyl cyclase activity My Citations
SL Sayner, R Balczon, DW Frank, DMF Cooper… – American Journal of …, 2011
Abstract Transmembrane adenylyl cyclase (AC) generates a cAMP pool within the
subplasma membrane compartment that strengthens the endothelial cell barrier. This cAMP
signal is steered toward effectors that promote junctional integrity and is inactivated before …
subplasma membrane compartment that strengthens the endothelial cell barrier. This cAMP
signal is steered toward effectors that promote junctional integrity and is inactivated before …
Genetic analysis of exoenzyme S expression by Pseudomonas aeruginosa My Citations
J Goranson, DW Frank – FEMS microbiology letters, 1996
Abstract The dissemination of Pseudomonas aeruginosa to the bloodstream increases the
likelihood of developing fatal sepsis. In experimental models, the ability to disseminate is
linked to expression of the exoenzyme S pathway. Genetic and biochemical analysis of …
likelihood of developing fatal sepsis. In experimental models, the ability to disseminate is
linked to expression of the exoenzyme S pathway. Genetic and biochemical analysis of …
Genetics and genetic manipulation in Francisella tularensis My Citations
DW Frank, TC Zahrt – Annals of the New York Academy of Sciences, 2007
Abstract: Francisella tularensis is a gram-negative coccobacillus and the etiological agent of
tularemia. The limited knowledge regarding the interaction of F. tularensis with its host is due
in part to the previous lack of tools for genetically manipulating the organism. During the …
tularemia. The limited knowledge regarding the interaction of F. tularensis with its host is due
in part to the previous lack of tools for genetically manipulating the organism. During the …
Children with cystic fibrosis produce an immune response against exoenzyme S, a type III cytotoxin of Pseudomonas aeruginosa My Citations
B Banwart, ML Splaingard, PM Farrell, MJ Rock… – Journal of Infectious …, 2002
To the Editor—In a recent article, Thio et al.[1] sought to determine whether DQB1* 0301 and
other HLA class II alleles were associated with hepatitis C virus (HCV) clearance and
persistence in 3 multiethnic cohorts. The results of their analyses confirmed the previously …
other HLA class II alleles were associated with hepatitis C virus (HCV) clearance and
persistence in 3 multiethnic cohorts. The results of their analyses confirmed the previously …
Functional analysis of exsC and exsB in regulation of exoenzyme S production by Pseudomonas aeruginosa. My Citations
J Goranson, AK Hovey, DW Frank – Journal of bacteriology, 1997
ABSTRACT Expression of ExsC, ExsB, and ExsA (the exoenzyme S trans-regulatory locus)
of Pseudomonas aeruginosa was analyzed by using complementation, RNase protection,
translational fusion, and T7-directed protein expression analyses. T7 expression analyses …
of Pseudomonas aeruginosa was analyzed by using complementation, RNase protection,
translational fusion, and T7-directed protein expression analyses. T7 expression analyses …
Isolation and characterization of monoclonal antibodies to Bordetella pertussis My Citations
DW Frank, CD Parker – Journal of biological standardization, 1984
Monoclonal antibodies to Bordelella pertussis were produced by fusion of mouse myeloma
cells and spleen cells of immunized mice. Cell lines were examined for specific antibody
production against several crude antigen preparations and lipopolysaccharide. Cross …
cells and spleen cells of immunized mice. Cell lines were examined for specific antibody
production against several crude antigen preparations and lipopolysaccharide. Cross …
Multi-functional characteristics of the Pseudomonas aeruginosa type III needle-tip protein, PcrV; comparison to orthologs in other Gram-negative bacteria My Citations
H Sato, DW Frank – Frontiers in microbiology, 2011
Pseudomonas aeruginosa possesses a type III secretion system (T3SS) to intoxicate host
cells and evade innate immunity. This virulence-related machinery consists of a molecular
syringe and needle assembled on the bacterial surface, which allows delivery ofT3 …
cells and evade innate immunity. This virulence-related machinery consists of a molecular
syringe and needle assembled on the bacterial surface, which allows delivery ofT3 …
Characterization of a gene that regulates toxin A synthesis in Pseudomonas aeruginosa My Citations
MS Hindahl, DW Frank, A Hamood, BH Iglewski – Nucleic acids research, 1988
The plasmid pFHKlO has been shown to contain a 3 kb Xhol fragment from PA103
chromosomal DNA which increases exotoxin A production (1). Subcloning and
complementation analysis indicated that the positive regulatory gene (regA) resided on a …
chromosomal DNA which increases exotoxin A production (1). Subcloning and
complementation analysis indicated that the positive regulatory gene (regA) resided on a …
Effect of regB on expression from the P1 and P2 promoters of the Pseudomonas aeruginosa regAB operon. My Citations
DG Storey, TL Raivio, DW Frank, MJ Wick, S Kaye… – Journal of bacteriology, 1991
ABSTRACT Exotoxin A production in Pseudomonas aeruginosa is dependent on two
regulatory genes, regA and regB, which are located in tandem on the chromosome.
Expression of regA and regB is controlled by two promoters (P1 and P2) situated …
regulatory genes, regA and regB, which are located in tandem on the chromosome.
Expression of regA and regB is controlled by two promoters (P1 and P2) situated …
Ubiquitin and ubiquitin‐modified proteins activate the Pseudomonas aeruginosa T3SS cytotoxin, ExoU My Citations
DM Anderson, KM Schmalzer, H Sato, M Casey… – Molecular microbiology, 2011
Summary Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that
possesses a type III secretion system (T3SS) critical for evading innate immunity and
establishing acute infections in compromised patients. Our research has focused on the …
possesses a type III secretion system (T3SS) critical for evading innate immunity and
establishing acute infections in compromised patients. Our research has focused on the …
Pseudolipasin A is a specific inhibitor for phospholipase A2 activity of Pseudomonas aeruginosa cytotoxin ExoU My Citations
VT Lee, S Pukatzki, H Sato, E Kikawada… – Infection and immunity, 2007
ABSTRACT A number of bacterial pathogens utilize the type III secretion pathway to deliver
effector proteins directly into the host cell cytoplasm. Certain strains of Pseudomonas
aeruginosa associated with acute infections express a potent cytotoxin, exoenzyme U ( …
effector proteins directly into the host cell cytoplasm. Certain strains of Pseudomonas
aeruginosa associated with acute infections express a potent cytotoxin, exoenzyme U ( …
Intracellular localization and processing of Pseudomonas aeruginosa ExoS in eukaryotic cells My Citations
KJ Pederson, S Pal, AJ Vallis, DW Frank, JT Barbieri – Molecular microbiology, 2000
Abstract ExoS is a type III cytotoxin of Pseudomonas aeruginosa, which modulates two
eukaryotic signalling pathways. The N-terminus (residues 1–234) is a GTPase activating
protein (GAP) for RhoGTPases, while the C-terminus (residues 232–453) encodes an …
eukaryotic signalling pathways. The N-terminus (residues 1–234) is a GTPase activating
protein (GAP) for RhoGTPases, while the C-terminus (residues 232–453) encodes an …
Molecular studies of a positive regulator of toxin A synthesis in Pseudomonas aeruginosa My Citations
MS Hindahl, DW Frank, BH Iglewski – 1987
Although the physical, biochemical, and physiological properties of a number of bacterial
toxins are well defined, the regulation of toxin produc tion at the molecular level is less than
well understood. Some insight into this subject has been provided by Weiss and Falkow [1 …
toxins are well defined, the regulation of toxin produc tion at the molecular level is less than
well understood. Some insight into this subject has been provided by Weiss and Falkow [1 …
Identification of regB, a gene required for optimal exotoxin A yields in Pseudomonas aeruginosa My Citations
MJ Wick, DW Frank, DG Storey, BH Iglewski – Molecular microbiology, 1990
Summary The yield of exotoxin A from Pseudomonas aeruginosa has been shown to be
strain-dependent. Exotoxin A production requires the presence of the positive regulatory
gene, regA. We cloned the regA genetic locus from the prototypical P. aeruginosa strain …
strain-dependent. Exotoxin A production requires the presence of the positive regulatory
gene, regA. We cloned the regA genetic locus from the prototypical P. aeruginosa strain …
Interaction of monoclonal antibodies with pertussis toxin and its subunits. My Citations
DW Frank, CD Parker – Infection and immunity, 1984
ABSTRACT The binding of two monoclonal antibodies to crude and pure preparations of
pertussis toxin was examined. Antibody P11B10 reacted with an epitope present on the S2
subunit of pertussis toxin by immunoblot techniques. Radioimmunoprecipitation analysis …
pertussis toxin was examined. Antibody P11B10 reacted with an epitope present on the S2
subunit of pertussis toxin by immunoblot techniques. Radioimmunoprecipitation analysis …
Cytotoxic Strains of Pseudotnonas aeruginosa Can Damage the Intact Corneal Surface In Vitro. My Citations
SMJ Fleiszig, EJ Lee, C Wu, RC Andika, V Valias… – Eye & Contact Lens, 1998
Purpose: Although the corneal epithelial cell layer is believed to serve as a barrier against
most types of bacteria, certain strains of P. aeruginosa have been shown to kill corneal
epithelial cells in primary cultures. The aim of this study was to test whether these strains …
most types of bacteria, certain strains of P. aeruginosa have been shown to kill corneal
epithelial cells in primary cultures. The aim of this study was to test whether these strains …
Identification, characterization and immunogenicity of an O-antigen capsular polysaccharide of Francisella tularensis My Citations
MA Apicella, DMB Post, AC Fowler, BD Jones… – PLoS One, 2010
Abstract Capsular polysaccharides are important factors in bacterial pathogenesis and have
been the target of a number of successful vaccines. Francisella tularensis has been
considered to express a capsular antigen but none has been isolated or characterized. …
been the target of a number of successful vaccines. Francisella tularensis has been
considered to express a capsular antigen but none has been isolated or characterized. …
Sera from adult patients with cystic fibrosis contain antibodies to Pseudomonas aeruginosa type III apparatus My Citations
J Moss, ME Ehrmantraut, BD Banwart, DW Frank… – Infection and immunity, 2001
ABSTRACT Expression of type III proteins of Pseudomonas aeruginosa in patients with
cystic fibrosis (CF) was investigated by measuring the immune response against
components of the type III pathway. Twenty-three of the 33 sera contained antibodies …
cystic fibrosis (CF) was investigated by measuring the immune response against
components of the type III pathway. Twenty-three of the 33 sera contained antibodies …
Multiple promoters control the regulation of the Pseudomonas aeruginosa regA gene My Citations
DG Storey, DW Frank, MA Farinha, AM Kropinski… – Molecular microbiology, 1990
Summary Expression studies utilizing the regA promoters, fused in tandem or separately to
promoterless reporter genes, indicated that regA is transcribed from two promoters (P1 and
P2). Both promoters can act independently. Expression from the P1 promoter is not …
promoterless reporter genes, indicated that regA is transcribed from two promoters (P1 and
P2). Both promoters can act independently. Expression from the P1 promoter is not …
Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU My Citations
H Sato, JB Feix, CJ Hillard, DW Frank – Journal of bacteriology, 2005
ABSTRACT Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro
phospholipase assay as a basis for identifying the mechanism for enzyme activation and
substrate specificity. Our results support a model in which a eukaryotic protein cofactor or …
phospholipase assay as a basis for identifying the mechanism for enzyme activation and
substrate specificity. Our results support a model in which a eukaryotic protein cofactor or …
Purification and characterization of exoenzyme S from Pseudomonas aeruginosa 388. My Citations
SM Kulich, DW Frank, JT Barbieri – Infection and immunity, 1993
ABSTRACT Exoenzyme S was purified> 1,500-fold from the culture supernatant fluid of
Pseudomonas aeruginosa 388 at high yield without utilization of solvents or detergents. Two
proteins, with apparent molecular sizes of 53 and 49 kDa, cofractionated with exoenzyme …
Pseudomonas aeruginosa 388 at high yield without utilization of solvents or detergents. Two
proteins, with apparent molecular sizes of 53 and 49 kDa, cofractionated with exoenzyme …
Expression of recombinant exoenzyme S of Pseudomonas aeruginosa. My Citations
SM Kulich, DW Frank, JT Barbieri – Infection and immunity, 1995
ABSTRACT The structural gene for the 49-kDa form of exoenzyme S (exoS) isolated from
Pseudomonas aeruginosa 388 was expressed in both Escherichia coli and P. aeruginosa
PA103. Expression of exoS in E. coli under the transcriptional regulation of the T7 …
Pseudomonas aeruginosa 388 was expressed in both Escherichia coli and P. aeruginosa
PA103. Expression of exoS in E. coli under the transcriptional regulation of the T7 …
A Francisella tularensis Schu S4 purine auxotroph is highly attenuated in mice but offers limited protection against homologous intranasal challenge My Citations
RD Pechous, TR McCarthy, NP Mohapatra, S Soni… – PLoS One, 2008
Background Francisella tularensis is a Gram-negative coccobacillus that causes the febrile
illness tularemia. Subspecies that are pathogenic for humans include those comprising the
type A (subspecies tularensis) or type B (subspecies holarctica) biovars. An attenuated …
illness tularemia. Subspecies that are pathogenic for humans include those comprising the
type A (subspecies tularensis) or type B (subspecies holarctica) biovars. An attenuated …
Interruption of Multiple Cellular Processes in HT-29 Epithelial Cells by Pseudomonas aeruginosaExoenzyme S My Citations
JC Olson, JE Fraylick, EM McGuffie, KM Dolan, TL Yahr… – Infection and immunity, 1999
ABSTRACT Exoenzyme S (ExoS), an ADP-ribosylating enzyme produced by the
opportunistic pathogen Pseudomonas aeruginosa, is directly translocated into eukaryotic
cells by bacterial contact. Within the cell, ExoS ADP-ribosylates the cell signaling protein …
opportunistic pathogen Pseudomonas aeruginosa, is directly translocated into eukaryotic
cells by bacterial contact. Within the cell, ExoS ADP-ribosylates the cell signaling protein …
Identification of superoxide dismutase as a cofactor for the pseudomonas type III toxin, ExoU My Citations
H Sato, JB Feix, DW Frank – Biochemistry, 2006
Pseudomonas aeruginosa is an opportunistic pathogen that uses a type III secretion system
and four effector proteins to avoid innate immune responses. ExoS, ExoT, ExoY, and ExoU
all possess enzymatic activities that disrupt host cellular physiology and prevent bacterial …
and four effector proteins to avoid innate immune responses. ExoS, ExoT, ExoY, and ExoU
all possess enzymatic activities that disrupt host cellular physiology and prevent bacterial …
Biochemical relationships between the 53-kilodalton (Exo53) and 49-kilodalton (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa. My Citations
S Liu, TL Yahr, DW Frank, JT Barbieri – Journal of bacteriology, 1997
ABSTRACT Genetic studies have shown that the 53-kDa (Exo53) and 49-kDa (ExoS) forms
of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT
and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid …
of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT
and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid …
PcrV immunization enhances survival of burned Pseudomonas aeruginosa-infected mice My Citations
IA Holder, AN Neely, DW Frank – Infection and immunity, 2001
ABSTRACT Burned Pseudomonas aeruginosa-infected mice immunized against PcrV, a
type III virulence system translocating protein, showed significantly enhanced survival
compared to controls. Survival was non-O serotype specific and correlated with a reduced …
type III virulence system translocating protein, showed significantly enhanced survival
compared to controls. Survival was non-O serotype specific and correlated with a reduced …
Multiple domains are required for the toxic activity of Pseudomonas aeruginosa ExoU My Citations
V Finck-Barbançon, DW Frank – Journal of bacteriology, 2001
ABSTRACT Expression of ExoU by Pseudomonas aeruginosa is correlated with acute
cytotoxicity in a number of epithelial and macrophage cell lines. In vivo, ExoU is responsible
for epithelial injury. The absence of a known motif or significant homology with other …
cytotoxicity in a number of epithelial and macrophage cell lines. In vivo, ExoU is responsible
for epithelial injury. The absence of a known motif or significant homology with other …
Acquisition and evolution of the exoU locus in Pseudomonas aeruginosa My Citations
BR Kulasekara, HD Kulasekara, MC Wolfgang… – Journal of bacteriology, 2006
ABSTRACT ExoU is a potent Pseudomonas aeruginosa cytotoxin translocated into host
cells by the type III secretion system. A comparison of genomes of various P. aeruginosa
strains showed that that the ExoU determinant is found in the same polymorphic region of …
cells by the type III secretion system. A comparison of genomes of various P. aeruginosa
strains showed that that the ExoU determinant is found in the same polymorphic region of …
Identification and characterization of SpcU, a chaperone required for efficient secretion of the ExoU cytotoxin My Citations
V Finck-Barbançon, TL Yahr, DW Frank – Journal of bacteriology, 1998
ABSTRACT In recent studies, we have shown that Pseudomonas aeruginosa strains that are
acutely cytotoxic in vitro damage the lung epithelium in vivo. Genetic analysis indicated that
the factor responsible for acute cytotoxicity was controlled by ExsA and therefore was part …
acutely cytotoxic in vitro damage the lung epithelium in vivo. Genetic analysis indicated that
the factor responsible for acute cytotoxicity was controlled by ExsA and therefore was part …
Type IV pili in Francisella tularensis: roles of pilF and pilT in fiber assembly, host cell adherence, and virulence My Citations
S Chakraborty, M Monfett, TM Maier, JL Benach… – Infection and immunity, 2008
ABSTRACT Francisella tularensis, a highly virulent facultative intracellular bacterium, is the
causative agent of tularemia. Genome sequencing of all F. tularensis subspecies revealed
the presence of genes that could encode type IV pili (Tfp). The live vaccine strain (LVS) …
causative agent of tularemia. Genome sequencing of all F. tularensis subspecies revealed
the presence of genes that could encode type IV pili (Tfp). The live vaccine strain (LVS) …
Construction and characterization of an attenuated purine auxotroph in a Francisella tularensis live vaccine strain My Citations
R Pechous, J Celli, R Penoske, SF Hayes, DW Frank… – Infection and immunity, 2006
ABSTRACT Francisella tularensis is a facultative intracellular pathogen and is the etiological
agent of tularemia. It is capable of escaping from the phagosome, replicating to high
numbers in the cytosol, and inducing apoptosis in macrophages of a variety of hosts. F. …
agent of tularemia. It is capable of escaping from the phagosome, replicating to high
numbers in the cytosol, and inducing apoptosis in macrophages of a variety of hosts. F. …
Kinetics of toxA and regA mRNA accumulation in Pseudomonas aeruginosa. My Citations
DW Frank, BH Iglewski – Journal of bacteriology, 1988
ABSTRACT DNA probes specific for an internal portion of the toxA and regA genes were
used to examine the synthesis of mRNA during the growth cycle of P. aeruginosa PA103.
RNA dot blot analysis revealed that in a low-iron growth medium, the synthesis of regA …
used to examine the synthesis of mRNA during the growth cycle of P. aeruginosa PA103.
RNA dot blot analysis revealed that in a low-iron growth medium, the synthesis of regA …
Type III secretion/intoxication system important in virulence of Pseudomonasaeruginosa infections in burns My Citations
IA Holder, AN Neely, DW Frank – Burns, 2001
Uncovering new virulence mechanisms by which microorganisms gain a foothold and
overcome host defenses provides us with opportunities to explore means to neutralize these
mechanisms and, thereby, enhance the host [2] resistance/defences against the …
overcome host defenses provides us with opportunities to explore means to neutralize these
mechanisms and, thereby, enhance the host [2] resistance/defences against the …
Differential regulation by iron of regA and toxA transcript accumulation in Pseudomonas aeruginosa. My Citations
DW Frank, DG Storey, MS Hindahl, BH Iglewski – Journal of bacteriology, 1989
ABSTRACT Iron regulation of toxA and regA transcript accumulation was examined in
Pseudomonas aeruginosa PA103 containing the regA gene on a multicopy plasmid. The
patterns of transcript accumulation for toxA and regA were found to be positively …
Pseudomonas aeruginosa PA103 containing the regA gene on a multicopy plasmid. The
patterns of transcript accumulation for toxA and regA were found to be positively …
Effects of differential expression of the 49-kilodalton exoenzyme S by Pseudomonas aeruginosa on cultured eukaryotic cells. My Citations
JC Olson, EM McGuffie, DW Frank – Infection and immunity, 1997
ABSTRACT Production of the ADP-ribosylating enzyme exoenzyme S (ExoS) by
Pseudomonas aeruginosa has been associated with increased virulence. Previous studies,
however, have been unable to confirm an effect of soluble ExoS in cell culture or animal …
Pseudomonas aeruginosa has been associated with increased virulence. Previous studies,
however, have been unable to confirm an effect of soluble ExoS in cell culture or animal …
Macrophages and epithelial cells respond differently to the Pseudomonas aeruginosa type III secretion system My Citations
J Coburn, DW Frank – Infection and immunity, 1999
ABSTRACT The multiple effects of Pseudomonas aeruginosa type III secretion have largely
been attributed to variations in cytotoxin expression between strains. Here we show that the
target cell type is also important. While lung epithelial cells showed significant changes in …
been attributed to variations in cytotoxin expression between strains. Here we show that the
target cell type is also important. While lung epithelial cells showed significant changes in …
Role of exoenzyme S in chronicPseudomonas aeruginosa lung infections My Citations
TI Nicas, DW Frank, P Stenzel, JD Lile, BH Iglewski – European journal of clinical …, 1985
Abstract Exoenzyme S is an extracellular ADP-ribosyltransferase enzyme produced by
Pseudomonas aeruginosa. Mutants of Pseudomonas aeruginosa deficient in this enzyme
have been shown to have reduced virulence in infections of burned mice. The contribution …
Pseudomonas aeruginosa. Mutants of Pseudomonas aeruginosa deficient in this enzyme
have been shown to have reduced virulence in infections of burned mice. The contribution …
Cloning the structural gene for the 49-kDa form of exoenzyme S (exoS) from Pseudomonas aeruginosa strain 388. My Citations
SM Kulich, TL Yahr, LM Mende-Mueller, JT Barbieri… – Journal of Biological …, 1994
Abstract We report the purification and proteolytic characterization of the 49-kDa form of
exoenzyme S and the cloning of the structural gene for the 49-kDa form of exoenzyme S
(exoS). The 49-kDa form of exoenzyme S was purified from SDS-polyacrylamide gels. …
exoenzyme S and the cloning of the structural gene for the 49-kDa form of exoenzyme S
(exoS). The 49-kDa form of exoenzyme S was purified from SDS-polyacrylamide gels. …
ExoT of Cytotoxic Pseudomonas aeruginosaPrevents Uptake by Corneal Epithelial Cells My Citations
BA Cowell, DY Chen, DW Frank, AJ Vallis… – Infection and immunity, 2000
ABSTRACT The presence of invasion-inhibitory activity that is regulated by the
transcriptional activator ExsA of cytotoxic Pseudomonas aeruginosa has previously been
proposed. The results of this study show that both ExoT and ExoS, known type III secreted …
transcriptional activator ExsA of cytotoxic Pseudomonas aeruginosa has previously been
proposed. The results of this study show that both ExoT and ExoS, known type III secreted …
Cloning and sequence analysis of a trans-regulatory locus required for exoenzyme S synthesis in Pseudomonas aeruginosa. My Citations
DW Frank, BH Iglewski – Journal of bacteriology, 1991
ABSTRACT Exoenzyme S is an ADP-ribosyltransferase enzyme distinct from exotoxin A that
is synthesized and secreted by Pseudomonas aeruginosa. Yields of exoenzyme S are
variable and depend on strain and growth conditions. Since certain medium additives are …
is synthesized and secreted by Pseudomonas aeruginosa. Yields of exoenzyme S are
variable and depend on strain and growth conditions. Since certain medium additives are …
In Vitro Cellular Toxicity PredictsPseudomonas aeruginosa Virulence in Lung Infections My Citations
T Sawa, M Ohara, K Kurahashi, SS Twining, DW Frank… – Infection and immunity, 1998
ABSTRACT The role of quorum sensing by Pseudomonas aeruginosa in producing
cytotoxicity has not been fully investigated. Strains ofP. aeruginosa have been characterized
as having an invasive or a cytotoxic phenotype (SMJ Fleiszig et al., Infect. Immun. 65: 579 …
cytotoxicity has not been fully investigated. Strains ofP. aeruginosa have been characterized
as having an invasive or a cytotoxic phenotype (SMJ Fleiszig et al., Infect. Immun. 65: 579 …
Generation and characterization of a protective monoclonal antibody to Pseudomonas aeruginosa PcrV My Citations
DW Frank, A Vallis, JP Wiener-Kronish… – Journal of Infectious …, 2002
Abstract Pseudomonas aeruginosa is a gram-negative pathogen causing life-threatening
infections. Lung injury and the development of sepsis depend largely on the expression of
type III secretion system (TTSS) virulence. TTSS functions as a molecular syringe to …
infections. Lung injury and the development of sepsis depend largely on the expression of
type III secretion system (TTSS) virulence. TTSS functions as a molecular syringe to …
Alveolar epithelial injury and pleural empyema in acute P. aeruginosa pneumonia in anesthetized rabbits My Citations
JP Wiener-Kronish, T Sakuma, I Kudoh, JF Pittet… – Journal of Applied …, 1993
Abstract We developed an experimental model of acute Pseudomonas aeruginosa
pneumonia in anesthetized ventilated rabbits to determine whether bacterial-induced injury
to the alveolar epithelium would occur and the effect of the injury on the pleural space. …
pneumonia in anesthetized ventilated rabbits to determine whether bacterial-induced injury
to the alveolar epithelium would occur and the effect of the injury on the pleural space. …
Transcriptional analysis of the Pseudomonas aeruginosa exoenzyme S structural gene. My Citations
TL Yahr, AK Hovey, SM Kulich, DW Frank – Journal of bacteriology, 1995
ABSTRACT The transcriptional regulation of the Pseudomonas aeruginosa exoS gene was
investigated. Expression of exoS in P. aeruginosa PA103 was dependent upon growth in a
low-cation environment and the presence of a functional exsA gene. Promoter fusion …
investigated. Expression of exoS in P. aeruginosa PA103 was dependent upon growth in a
low-cation environment and the presence of a functional exsA gene. Promoter fusion …
Effect of Pseudomonas aeruginosa elastase, alkaline protease, and exotoxin A on corneal proteinases and proteins. My Citations
SS Twining, SE Kirschner, LA Mahnke, DW Frank – Investigative ophthalmology & …, 1993
PURPOSE: To determine the effects of exoproducts from the corneal pathogen
Pseudomonas aeruginosa on corneal proteinases and proteins. METHODS: Whole rabbit
corneas were cultured in the presence or absence of broths conditioned with …
Pseudomonas aeruginosa on corneal proteinases and proteins. METHODS: Whole rabbit
corneas were cultured in the presence or absence of broths conditioned with …
Characterization of Pseudomonas aeruginosa-induced MDCK cell injury: glycosylation-defective host cells are resistant to bacterial killing. My Citations
G Apodaca, M Bomsel, R Lindstedt, J Engel, D Frank… – Infection and immunity, 1995
ABSTRACT As a model for bacterium-induced epithelial cell injury, we have studied the
interaction of Pseudomonas aeruginosa with polarized Madin-Darby canine kidney (MDCK)
cells grown on filters. Following an initial period of bacterial adhesion, foci of injured host …
interaction of Pseudomonas aeruginosa with polarized Madin-Darby canine kidney (MDCK)
cells grown on filters. Following an initial period of bacterial adhesion, foci of injured host …
Pseudomonas aeruginosa exoenzyme S ADP-ribosylates Ras at multiple sites My Citations
AK Ganesan, DW Frank, RP Misra, G Schmidt… – Journal of Biological …, 1998
Abstract Pseudomonas aeruginosa exoenzyme S (ExoS) ADP-ribosylated Ras to a
stoichiometry of∼ 2 molecules of ADP-ribose incorporated per molecule of Ras, which
suggested that ExoS could ADP-ribosylate Ras at more than one arginine residue. SDS- …
stoichiometry of∼ 2 molecules of ADP-ribose incorporated per molecule of Ras, which
suggested that ExoS could ADP-ribosylate Ras at more than one arginine residue. SDS- …
Construction and characterization of a highly efficient Francisella shuttle plasmid My Citations
TM Maier, A Havig, M Casey, FE Nano, DW Frank… – Applied and environmental …, 2004
ABSTRACT Francisella tularensis is a facultative intracellular pathogen that infects a wide
variety of mammals and causes tularemia in humans. It is recognized as a potential agent of
bioterrorism due to its low infectious dose and multiple routes of transmission. To date, …
variety of mammals and causes tularemia in humans. It is recognized as a potential agent of
bioterrorism due to its low infectious dose and multiple routes of transmission. To date, …
Genetic relationship between the 53-and 49-kilodalton forms of exoenzyme S from Pseudomonas aeruginosa. My Citations
TL Yahr, JT Barbieri, DW Frank – Journal of bacteriology, 1996
ABSTRACT Exoenzyme S is an ADP-ribosylating extracellular protein of Pseudomonas
aeruginosa that is produced as two immunologically related forms, a 49-kDa enzymatically
active form and a 53-kDa inactive form. The postulated relationship between the two …
aeruginosa that is produced as two immunologically related forms, a 49-kDa enzymatically
active form and a 53-kDa inactive form. The postulated relationship between the two …
Exoproduct secretions of Pseudomonas aeruginosa strains influence severity of alveolar epithelial injury My Citations
I Kudoh, JP Wiener-Kronish, S Hashimoto, JF Pittet… – American Journal of …, 1994
Abstract To determine whether exoenzyme S plays a role in alveolar epithelial injury, two
parental strains of Pseudomonas aeruginosa, PAK and PA103, were tested that produced
large quantities of exoenzyme S. Strains PAK and PA103 differ in the form of exoenzyme …
parental strains of Pseudomonas aeruginosa, PAK and PA103, were tested that produced
large quantities of exoenzyme S. Strains PAK and PA103 differ in the form of exoenzyme …
Analyses of the DNA-binding and transcriptional activation properties of ExsA, the transcriptional activator of the Pseudomonas aeruginosa exoenzyme S regulon. My Citations
AK Hovey, DW Frank – Journal of bacteriology, 1995
ABSTRACT ExsA has been implicated as a central regulator of exoenzyme S production by
Pseudomonas aeruginosa. In this study, the DNA-binding and transcriptional activation
properties of ExsA were investigated. ExsA was produced and purified as a fusion protein, …
Pseudomonas aeruginosa. In this study, the DNA-binding and transcriptional activation
properties of ExsA were investigated. ExsA was produced and purified as a fusion protein, …
Biological effects of Pseudomonas aeruginosa type III-secreted proteins on CHO cells My Citations
AJ Vallis, V Finck-Barbançon, TL Yahr, DW Frank – Infection and immunity, 1999
ABSTRACT A strain of Pseudomonas aeruginosa that fails to express known type III-
secreted effector proteins was constructed as an expression host. Individual effectors were
expressed intrans, and their biological effects on CHO cells were assessed in an acute …
secreted effector proteins was constructed as an expression host. Individual effectors were
expressed intrans, and their biological effects on CHO cells were assessed in an acute …
Regulation of ExoS Production and Secretion byPseudomonas aeruginosa in Response to Tissue Culture Conditions My Citations
AJ Vallis, TL Yahr, JT Barbieri, DW Frank – Infection and immunity, 1999
ABSTRACT This study was initiated to characterize the regulation and secretion of ExoS by
Pseudomonas aeruginosa during contact with eukaryotic cells. The production of ExoS was
monitored by a sensitive ADP-ribosyltransferase activity assay, and specific activities were …
Pseudomonas aeruginosa during contact with eukaryotic cells. The production of ExoS was
monitored by a sensitive ADP-ribosyltransferase activity assay, and specific activities were …
The amino‐terminal domain of Pseudomonas aeruginosa ExoS disrupts actin filaments via small‐molecular‐weight GTP‐binding proteins My Citations
KJ Pederson, AJ Vallis, K Aktories, DW Frank… – Molecular microbiology, 1999
Abstract Pseudomonas aeruginosa delivers exoenzyme S (ExoS) into the intracellular
compartment of eukaryotic cells via a type III secretion pathway. Intracellular delivery of
ExoS is cytotoxic for eukaryotic cells and has been shown to ADP-ribosylate Ras in vivo …
compartment of eukaryotic cells via a type III secretion pathway. Intracellular delivery of
ExoS is cytotoxic for eukaryotic cells and has been shown to ADP-ribosylate Ras in vivo …
Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis My Citations
S Miyata, M Casey, DW Frank, FM Ausubel, E Drenkard – Infection and immunity, 2003
ABSTRACT Nonvertebrate model hosts represent valuable tools for the study of host-
pathogen interactions because they facilitate the identification of bacterial virulence factors
and allow the discovery of novel components involved in host innate immune responses. …
pathogen interactions because they facilitate the identification of bacterial virulence factors
and allow the discovery of novel components involved in host innate immune responses. …
ExoU is a potent intracellular phospholipase My Citations
H Sato, DW Frank – Molecular microbiology, 2004
Summary The combination of a large genome encoding metabolic versatility and conserved
secreted virulence determinants makes Pseudomonas aeruginosa a model pathogen that
can be used to study host–parasite interactions in many eukaryotic hosts. One of the …
secreted virulence determinants makes Pseudomonas aeruginosa a model pathogen that
can be used to study host–parasite interactions in many eukaryotic hosts. One of the …
Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon. My Citations
TL Yahr, LM Mende-Mueller, MB Friese, DW Frank – Journal of bacteriology, 1997
ABSTRACT Extracellular protein profiles from wild-type and regulatory or secretory isogenic
mutants of the Pseudomonas aeruginosa exoenzyme S regulon were compared to identify
proteins coordinately secreted with ExoS. Data from amino-terminal sequence analysis of …
mutants of the Pseudomonas aeruginosa exoenzyme S regulon were compared to identify
proteins coordinately secreted with ExoS. Data from amino-terminal sequence analysis of …
Pseudomonas aeruginosa-mediated cytotoxicity and invasion correlate with distinct genotypes at the loci encoding exoenzyme S. My Citations
SM Fleiszig, JP Wiener-Kronish, H Miyazaki, V Vallas… – Infection and immunity, 1997
ABSTRACT Pseudomonas aeruginosa, an opportunistic pathogen, is capable of
establishing both chronic and acute infections in compromised hosts. Previous studies
indicated that P. aeruginosa displays either a cytotoxic or an invasive phenotype in …
establishing both chronic and acute infections in compromised hosts. Previous studies
indicated that P. aeruginosa displays either a cytotoxic or an invasive phenotype in …
The mechanism of action of the Pseudomonas aeruginosa‐encoded type III cytotoxin, ExoU My Citations
H Sato, DW Frank, CJ Hillard, JB Feix, RR Pankhaniya… – The EMBO Journal, 2003
Abstract Pseudomonas aeruginosa delivers the toxin ExoU to eukaryotic cells via a type III
secretion system. Intoxication with ExoU is associated with lung injury, bacterial
dissemination and sepsis in animal model and human infections. To search for ExoU …
secretion system. Intoxication with ExoU is associated with lung injury, bacterial
dissemination and sepsis in animal model and human infections. To search for ExoU …
Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway My Citations
TL Yahr, J Goranson, DW Frank – Molecular microbiology, 1996
Abstract Exoenzyme S is an extracellular ADP-ribosyltransferase of Pseudomonas
aeruginosa. Transposon mutagenesis of P. aeruginosa 388 was used to identify genes
required for exoenzyme S production. Five Tn5 Tc insertion mutants were isolated which …
aeruginosa. Transposon mutagenesis of P. aeruginosa 388 was used to identify genes
required for exoenzyme S production. Five Tn5 Tc insertion mutants were isolated which …
Active and passive immunization with the Pseudomonas V antigen protects against type III intoxication and lung injury My Citations
T Sawa, TL Yahr, M Ohara, K Kurahashi, MA Gropper… – Nature medicine, 1999
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that can cause fatal acute
lung infections in critically ill individuals. Damage to the lung epithelium is associated with
the expression of toxins that are directly injected into eukaryotic cells through a type III- …
lung infections in critically ill individuals. Damage to the lung epithelium is associated with
the expression of toxins that are directly injected into eukaryotic cells through a type III- …
Pathogenesis of septic shock in Pseudomonas aeruginosa pneumonia My Citations
K Kurahashi, O Kajikawa, T Sawa, M Ohara… – The Journal of clinical …, 1999
Nosocomial pneumonia is a leading cause of mortality among critically ill patients (1–3). The
most common gram-negative organism associated with nosocomial pneumonia is
Pseudomonas aeruginosa (4–6). Patients with P. aeruginosa pneumonia are also more …
most common gram-negative organism associated with nosocomial pneumonia is
Pseudomonas aeruginosa (4–6). Patients with P. aeruginosa pneumonia are also more …
The exoenzyme S regulon of Pseudomonas aeruginosa My Citations
DW Frank – Molecular microbiology, 1997
Abstract Pseudomonas aeruginosa can cause severe life-threatening infections in which the
bacterium disseminates rapidly from epithelial colonization sites to the bloodstream. In
experimental models, the ability of P. aeruginosa to disseminate is linked to epithelial …
bacterium disseminates rapidly from epithelial colonization sites to the bloodstream. In
experimental models, the ability of P. aeruginosa to disseminate is linked to epithelial …
Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections My Citations
A Roy-Burman, RH Savel, S Racine, BL Swanson… – Journal of Infectious …, 2001
Abstract The ability of Pseudomonas aeruginosa to secrete specific toxins using the type III–
mediated pathway has been reported. To determine the association of this phenotype with
human illness, immunoblot analysis was used to detect expression of type III secretory …
mediated pathway has been reported. To determine the association of this phenotype with
human illness, immunoblot analysis was used to detect expression of type III secretory …
ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system My Citations
TL Yahr, AJ Vallis, MK Hancock, JT Barbieri… – Proceedings of the …, 1998
Abstract The exoenzyme S regulon is a set of coordinately regulated virulence genes of
Pseudomonas aeruginosa. Proteins encoded by the regulon include a type III secretion and
translocation apparatus, regulators of gene expression, and effector proteins. The effector …
Pseudomonas aeruginosa. Proteins encoded by the regulon include a type III secretion and
translocation apparatus, regulators of gene expression, and effector proteins. The effector …
ExoU expression by Pseudomonas aeruginosa correlates with acute cytotoxicity and epithelial injury My Citations
V Finck‐Barbançon, J Goranson, L Zhu, T Sawa… – Molecular microbiology, 1997
Abstract The production of exoenzyme S is correlated with the ability of Pseudomonas
aeruginosa to disseminate from epithelial colonization sites and cause a fatal sepsis in burn
injury and acute lung infection models. Exoenzyme S is purified from culture supernatants …
aeruginosa to disseminate from epithelial colonization sites and cause a fatal sepsis in burn
injury and acute lung infection models. Exoenzyme S is purified from culture supernatants …
Sexual experience promotes adult neurogenesis in the hippocampus despite an initial elevation in stress hormones
B Leuner, ER Glasper, E Gould – PLoS One, 2010
Abstract Aversive stressful experiences are typically associated with increased anxiety and a
predisposition to develop mood disorders. Negative stress also suppresses adult
neurogenesis and restricts dendritic architecture in the hippocampus, a brain region …
predisposition to develop mood disorders. Negative stress also suppresses adult
neurogenesis and restricts dendritic architecture in the hippocampus, a brain region …