Recombinant human cardiac troponin T (cTnT)

Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3

Expressed in E. coli

Product of gene TNNT2; 288 a.a.r.
MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN ММHFGGYIQK QAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK
Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).

Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard

Purity > 95 % (SDS-PAGE).
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) – 0.490 (measured at 280 nm)

Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform

Immunoaffinity purification followed by ion-exchange chromatography

Lyophilized from 0.01 M HCl

Reconstitute with Tris/urea buffer (20 mM Tris pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol.

Lyophilized -20 °C (between -15 and -30 °C allowed)
Reconstituted -70 °C (between -65 and -80 °C allowed)

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.