Neuron-specific enolase (NSE)

Post mortal human brain.
Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.

Purity > 95 % (SDS-PAGE).

Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-Dglycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.

Tested in ELISA and immunoblotting with anti-NSE antibodies.

Ion-exchange chromatography and gel-filtration.

Presentation: Solution in 0.1 M Tris-HCl, 5 mM Mg2SO4, 0.25 M KCl, pH 5.1 adjusted with sodium phosphate.

-20 °C (between -15 and -30 °C allowed)

Avoid repeated freezing and thawing.

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.