Monoclonal mouse anti- human complex transferrin-transferrin receptor

1C10, 8B9, 11D3, 12A6
Hybridoma clones for MAbs 1C10 have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified transferrin from pooled human plasma.
Hybridoma clones for MAbs 8B9, 11D3 and 12A6 have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with placental transferrin receptor or transferrin.

Human transferrin for all MAbs.
Complex between transferrin and soluble transferrin receptor for MAbs 8B9 and 11D3.
MAbs 1C10 do not cross-react with mouse, rabbit, bovine transferrin and with human lactoferrin.

IgG1 for all MAbs

Detection of transferrin in serum by sandwich immunoassay. Detection of transferrin – transferrin receptor complex by sandwich ELISA. All MAbs are working in ELISA.
Recommended pairs for transferrin detection by sandwich immunoassay (capture-detection):
8B9 – 11D3
8B9 – 12A6
1C10 – 12A6
11D3 – 1C10
11D3 – 8B9
Recommended MAbs for transferrin-transferrin receptor complex detection by ELISA sandwich method using anti-transferrin receptor MAb 23D10 (Cat. # 2-TRs) as coating:
23D10 – 8B9, 23D10 – 11D3
At 10-6 M MAbs 1C10 inhibit proliferation of Con-A stimulated peripheral blood
lymphocytes.

Chromatography on protein A Sepharose for MAbs 8B9, 11D3 and 12A6, and chromatography on protein G Sepharose for MAbs 1C10

PBS, pH 7.4, 0.1 % sodium azide (NaN3)

+ 4 °C

This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Transferrin-Transferrin Receptor Complex Assay Notes