Monoclonal mouse anti-cardiac Troponin T (cTnT), in vitro

Catalog Number: 2-Ttc-iv

From: $310.00

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General Category:

    Monoclonal antibodies » Veterinary » Other animal specific antibodies
    Monoclonal antibodies » Cardiac Markers » Troponin T

Disease Category:

    Veterinary » Monoclonal antibodies » Other animal specific antibodies
    Cardiac Markers » Monoclonal antibodies » Troponin T
MAbs:
300cc, 329cc, 406cc, 1C11cc, 1F11cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic human TnT peptide a.a.r. 119-138 conjugated with carrier protein (for MAbs 300cc and 329cc), or synthetic human TnT peptide a.a.r. 106-183 conjugated with carrier protein (for MAb 406cc), or free human cTnT (MAbs 1C11cc and 1F11cc).
Specificity:
MAbs 406cc, 1C11cc and 1F11cc have no cross-reaction with skeletal TnT. Cross-reactivity of MAbs 300cc and 329cc with skeletal TnT is less than 1%.
MAb Isotypes:

IgG1 for MAb 300cc, 329cc, 1C11cc

IgG2a for MAb 406cc

IgG2b for MAb 1F11cc

Applications:

TnT immunoassay. All MAbs recognize TnT in sandwich immunoassay.

Recommended pairs for sandwich immunoassay (capture – detection):
406cc – 300cc
329cc – 406cc

MAbs 1C11cc and 1F11cc are working in Western blotting.

Purification:
Chromatography on protein A Sepharose.
Presentation:
PBS, pH 7.4, 0.09 % sodium azide (NaN3)
Storage Conditions:
+4 °C (+2 … +8 °C allowed)
Material Safety Note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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