Monoclonal mouse anti- canine N-terminal proBNP (NT-proBNP)

CaNT89, CaNT90*, CaNT19, CaNT46, CaNT49, CaNT53*
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with:
 recombinant canine NT-proBNP with tag expressed in E. coli (CaNT90)
 recombinant canine NT-proBNP expressed in E. coli as a fusion protein with carrier (CaNT89, CaNT19, CaNT46, CaNT925)
 synthetic peptides corresponding to 3-26 a.a.r., 13-33 a.a.r., and 64- 80 a.a.r. (CaNT49, CaNT53) of canine NT-proBNP

Canine NT-proBNP and proBNP.

IgG1 for MAbs CaNT89, CaNT90, CaNT19, CaNT46, CaNT49, CaNT53

Quantification of canine NT-proBNP in plasma of dogs. Recommended pairs for sandwich immunoassay (capture-detection):
CaNT90 – CaNT89
CaNT19 – CaNT89
CaNT90 – CaNT53.
Extended list of two-site antibody combinations for detection of canine NT-proBNP in plasma can be provided as a supplementary material.

Chromatography on protein A Sepharose

PBS, pH 7.4, 0.1 % sodium azide (NaN3)

+ 4 °C

This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
* Some applications in which these products may be used are covered by patents issued and applicable in certain countries. Because purchase of these products does not include a license to perform any patented application, users of these products may be required to obtain a patent license, depending on the particular application and country in which the product is used.

Canine NT-proBNP Assay Notes