Human recombinant NT-proBNP (amino terminal human brain natriuretic peptide precursor)

Recombinant E.coli.

Calibrator or standard in immunoassays, immunogen for antibody production.

Purity > 95 % (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.
Predicted molecular mass 8589 Da confirmed by MALDI-MS.
Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).

Immunoaffinity purification followed by ion-exchange chromatography.

10 mM potassium phosphate, pH 7.4, 150 mM NaCl

-70 °C (between -65 and -80 °C allowed)

Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes.
Antibodies for New Type of BNP immunoassay – Single Epitope Sandwich Assay Notes.

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